Frequently asked questions (FAQs)
Caprico Biotechnologies, Inc. manufactures and conjugates high-quality primary and secondary antibodies destined for use in several different clinical and research applications like flow cytometry, Western blot, ELISA, etc.
Our catalogue is mostly comprised of monoclonal antibodies directed against human CD antigens. In addition, we offer several antibodies directed against non-CD targets like p53 and ETS1, and several conjugated donkey-anti-mouse secondary antibodies.
Besides being available as purified antibodies, all of our Flow cytometry antibodies are available in a large variety of conjugates. Along with long time used fluorophores like fluorescein isothiocyanate (FITC), R-Phycoerythrin (PE) and Peridinin chlorophyll (PerCP), Caprico Biotechnologies offers fluorescent conjugates of the optimized mFluor® and iFluor® series. Please see our catalogue for our complete conjugation portfolio.
Please note that each product has its own application as listed in the TDS. We continue working to expand our inventory to include further fluorochromes, dyes and tandem dyes.
All conjugated antibodies are available as free test samples at 10 tests per tube size. In addition, Caprico Biotechnologies offers discounts and promotions on a regular basis. For detailed information, please check our website or contact our Customer Service Department at (678) 691-2143 Ext. 101.
The following is a list of common questions regarding the technical specifications and applications of our products.
All antibodies directed against human CD antigens are validated for use in flow cytometry. Please view the TDS for your specific product to verify which other applications your antibody is suitable for, as well as storage conditions, QC data, and much more.
Unless noted otherwise in the TDS, all conjugated antibodies should be stored at 4°C. The TDS for the product in question will contain the information outlining the appropriate storage conditions. It should be noted that conjugated antibodies should never be frozen, if possible, unless otherwise noted by that vendor's specifications.
Caprico Biotechnologies offers multiple antibody clones which are cross-reactive with different non-human primate (NHP) species. Species reactivity and conjugates tested can be found in the respective TDS, some data are available in the resources section. For detailed information about our NHP reactive products and the possibility of obtaining free product in exchange for test data, please contact our technical support.
Our technical support can be reached under (678) 691-2143 Ext. 102.
Flow Cytometry FAQs
The recommended test volume is indicated on the outer label of the product and on the TDS for that product. We recommend that every lab carries out an initial titration study before running your samples to ensure that the optimal concentration is selected for your application.
The isotype of any of our antibodies can be viewed in the "Isotype" field next to the clone name. Also, on the TDS for any given antibody, the "Host/Isotype" field will inform you about the isotype of that particular antibody. Once the correct isotype has been identified, just type "Isotype Control" or similar text into the search bar on the webpage and select the appropriate isotype control with the correct format (purified, FITC, PE, PerCP, etc.)
It is always best to use fresh samples, however there are measures you can take to preserve your samples for a later date. After completing your staining protocol, incubate your samples in fresh 1% paraformaldehyde for 5 to 10 minutes at room temperature. After incubation is complete, place your samples at 4°C without light exposure for up to 3 days.