Introduction This protocol describes the procedure for labeling cells using fresh whole blood. Keep in mind that in addition to antibodies detecting the cell markers of interest, matching isotype and FMO (fluorescence minus one) controls need to be included in… Read More
Protocol II: Indirect cell labeling for flow cytometry
Introduction This protocol describes the procedure for labeling cells using purified and Biotin conjugated antibodies. It can be applied to a large variety of cell types. Keep in mind that in addition to antibodies detecting the cell markers of interest,… Read More
Protocol I: Direct cell labeling for flow cytometry
Introduction This protocol describes the procedure for labeling cells using fluorophore-conjugated antibodies. It can be applied to a large variety of cell types. Keep in mind that in addition to antibodies detecting the cell markers of interest, matching isotype and… Read More
From Idea to Antibody