ZAP-70 PE
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Lymphocytes gated PMA stimulated fixed and permeabilized PBMCs intracellularly stained with ZAP70 (C-D12) PE (RED); Lymphocytes gated PMA stimulated fixed and permeabilized PBMCs are used as FMO control (BLUE).
| Conjugation | PE |
|---|---|
| Antigen | ZAP-70 |
| Clone | C-D12 |
| Isotype | IgG1, k |
| Applications | FC |
| Reactivity | Human |
| Storage | 2-8°C |
| Clonality | Monoclonal |
| Primary/Secondary | Primary |
| Format | 0.09% sodium azide, 0.2% BSA (w/v), PBS pH 7.2 |
| Status | RUO |
| Excitation Laser | Blue (488nm) |
Description:
The clone C-D12, a mouse monoclonal antibody selectively binds with the 70kd tyrosine phosphoprotein known as ZAP-70 which is usually associated with the ζ-chain CD3-receptor-associated protein tyrosine kinase (PTK). It is a member of the Syk family that is localized exclusively to the cytosol of T cells and natural killer (NK) cells and is required for their cellular activation. It is generally considered the T-lymphocyte counterpart of Syk, a B-cell receptor (BCR)-associated kinase that belongs to the same PTK family and plays a similar role in the antigen receptor signaling in the B-lineage cells. It facilitates the upregulation of Fas ligand in activation-induced T cell apoptosis. ZAP-70 expression plays an important role in the clinical diagnosis of various types of hematologic malignancies.
The clone C-D12, a mouse monoclonal antibody selectively binds with the 70kd tyrosine phosphoprotein known as ZAP-70 which is usually associated with the ζ-chain CD3-receptor-associated protein tyrosine kinase (PTK). It is a member of the Syk family that is localized exclusively to the cytosol of T cells and natural killer (NK) cells and is required for their cellular activation. It is generally considered the T-lymphocyte counterpart of Syk, a B-cell receptor (BCR)-associated kinase that belongs to the same PTK family and plays a similar role in the antigen receptor signaling in the B-lineage cells. It facilitates the upregulation of Fas ligand in activation-induced T cell apoptosis. ZAP-70 expression plays an important role in the clinical diagnosis of various types of hematologic malignancies.
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