New products for PNH clinical research!!!
Paroxysmal nocturnal hemoglobinuria (PNH) is a stem cell disorder caused by a mutation of the gene involved in the synthesis of the GPI (glycosylphosphatidylinositol) anchor of a group of surface proteins on circulating cells. Laboratory findings in PNH include typical findings of hemolytic anemia, loss of GPI-anchored proteins.
Flow cytometry will show reduced levels of GPI-anchored proteins on peripheral blood cells.
ASR-grade FLAER (Fluorescein-labeled Proaerolysin), along with antibodies CD45, CD55, CD59, CD14 and CD15, can be used to detect PNH clones (FLAER-negative cells) within the monocytes and granulocytes lineages in the application of multi-color flow cytometry analysis.
The analytical combination of CBI CD55 & CD59 assays can be used for detecting PHN clones in red blood cells. Flow Cytometry labs evaluate their own verified and optimized assay set up.
We are happy to provide you with free samples for your internal evaluation!
Great Performance Panel:
FLAER iFluor™488, CD59 PE, CD45 PerCP, CD55 APC
Fig. 1. The common PNH flow panel is established by using Caprico products (FLAER iFluor™488, CD59 PE, CD45 PerCP, CD55 APC) in RBC-lysed fresh blood from healthy donors.
FLAER iFluor™488 & FLAER mFluor™450 both are available right now!
Fig.2. Flow data of FLAER iFluor™488 (Right red histogram) and FLAER mFluor™450(Left red histogram) of lymphocytes gated populations. Unstained lymphocytes are used as FMO control (blue histogram).
Related products of multi-color flow panels are also available, for further information please check links below:
|CD55||CD14||CD33||CD64||10×RBC Lysis buffer|
*ASR: Analyte Specific Reagent. The analytical and performance characteristics of this ASR product are not established.